Detection of Pancreatic Cancer miRNA with Biocompatible Nitrogen-Doped Graphene Quantum Dots

Ajgaonkar, Ryan; Lee, Bong; Valimukhametova, Alina; Nguyen, Steven; Gonzalez-Rodriguez, Roberto; Coffer, Jeffery; Akkaraju, Giridhar R.; Naumov, Anton V.

Publication

Detection of Pancreatic Cancer miRNA with Biocompatible Nitrogen-Doped Graphene Quantum Dots

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Ajgaonkar, Ryan
Lee, Bong
Valimukhametova, Alina
Nguyen, Steven
Gonzalez-Rodriguez, Roberto
Coffer, Jeffery
Akkaraju, Giridhar R.
Naumov, Anton V.

Publisher

MDPI AG

Date

2022

Abstract

Early-stage pancreatic cancer remains challenging to detect, leading to a poor five-year patient survival rate. This obstacle necessitates the development of early detection approaches based on novel technologies and materials. In this work, the presence of a specific pancreatic cancer-derived miRNA (pre-miR-132) is detected using the fluorescence properties of biocompatible nitrogen-doped graphene quantum dots (NGQDs) synthesized using a bottom-up approach from a single glucosamine precursor. The sensor platform is comprised of slightly positively charged (1.14 ± 0.36 mV) NGQDs bound via ??? stacking and/or electrostatic interactions to the negatively charged (?22.4 ± 6.00 mV) bait ssDNA; together, they form a complex with a 20 nm average size. The NGQDs’ fluorescence distinguishes specific single-stranded DNA sequences due to bait–target complementarity, discriminating them from random control sequences with sensitivity in the micromolar range. Furthermore, this targetability can also detect the stem and loop portions of pre-miR-132, adding to the practicality of the biosensor. This non-invasive approach allows cancer-specific miRNA detection to facilitate early diagnosis of various forms of cancer.