This item appears in the following Collection(s)
- Research Publications [837]
Title | Graphene Oxide as a Multifunctional Platform for Intracellular Delivery, Imaging, and Cancer Sensing |
---|---|
Author | Campbell, Elizabeth; Hasan, Md. Tanvir; Pho, Christine; Callaghan, K.; Akkaraju, Giridhar R.; Naumov, Anton V. |
Date | 2019-01-23 |
Abstract | Graphene oxide (GO), the most common derivative of graphene, is an exceptional nanomaterial that possesses multiple physical properties critical for biomedical applications. GO exhibits pH-dependent fluorescence emission in the visible/near-infrared, providing a possibility of molecular imaging and pH-sensing. It is also water soluble and has a substantial platform for functionalization, allowing for the delivery of multiple therapeutics. GO physical properties are modified to enhance cellular internalization, producing fluorescent nanoflakes with low (<15%) cytotoxicity at the imaging concentrations of 15 µg/mL. As a result, at lower flake sizes GO rapidly internalizes into HeLa cells with the following 70% fluorescence based clearance at 24 h, assessed by its characteristic emission in red/near-IR. pH-dependence of GO emission is utilized to provide the sensing of acidic extracellular environments of cancer cells. The results demonstrate diminishing green/red (550/630 nm) fluorescence intensity ratios for HeLa and MCF-7 cancer cells in comparison to HEK-293 healthy cells suggesting a potential use of GO as a non-invasive optical sensor for cancer microenvironments. The results of this work demonstrate the potential of GO as a novel multifunctional platform for therapeutic delivery, biological imaging and cancer sensing. |
Link | https://doi.org/10.1038/s41598-018-36617-4
https://repository.tcu.edu/handle/116099117/26378 https://www.nature.com/articles/s41598-018-36617-4 |
Department | Physics and Astronomy Biology |
Subject | Drug delivery
Imaging techniques and agents Nanotechnology in cancer |