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dc.creatorDuggal, Divya
dc.creatorRequena, Sebastian
dc.creatorNagwekar, Janhavi
dc.creatorRaut, Sangram L.
dc.creatorRich, Ryan M.
dc.creatorDas, Hriday
dc.creatorPatel, Vipul
dc.creatorGryczynski, Ignacy
dc.creatorFudala, Rafal
dc.creatorGryczynski, Zygmunt
dc.creatorBlair, Cheavar
dc.creatorCampbell, Kenneth S.
dc.creatorBorejdo, Julian
dc.description.abstractThe systemic circulation offers larger resistance to the blood flow than the pulmonary system. Consequently, the left ventricle (LV) must pump blood with more force than the right ventricle (RV). The question arises whether the stronger pumping action of the LV is due to a more efficient action of left ventricular myosin, or whether it is due to the morphological differences between ventricles. Such a question cannot be answered by studying the entire ventricles or myocytes because any observed differences would be wiped out by averaging the information obtained from trillions of myosin molecules present in a ventricle or myocyte. We therefore searched for the differences between single myosin molecules of the LV and RV of failing hearts In-situ. We show that the parameters that define the mechanical characteristics of working myosin (kinetic rates and the distribution of spatial orientation of myosin lever arm) were the same in both ventricles. These results suggest that there is no difference in the way myosin interacts with thin filaments in myocytes of failing hearts, and suggests that the difference in pumping efficiencies are caused by interactions between muscle proteins other than myosin or that they are purely morphological.
dc.sourceFrontiers in Physiology
dc.subjectcross-bridge orientation
dc.subjectheart ventricles
dc.subjectfluorescence polarization
dc.titleNo Difference in Myosin Kinetics and Spatial Distribution of the Lever Arm in the Left and Right Ventricles of Human Hearts
dc.rights.holderDuggal et al.
dc.rights.licenseCC BY 4.0
local.collegeCollege of Science and Engineering
local.departmentPhysics and Astronomy
local.personsRaut, Z Gryczynski (PHYS)

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