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dc.creatorKhazi-Syed, Afeefah
dc.creatorHasan, Md. Tanvir
dc.creatorCampbell, Elizabeth
dc.creatorGonzalez-Rodriguez, Roberto
dc.creatorNaumov, Anton V.
dc.date.accessioned2020-05-11T16:12:56Z
dc.date.available2020-05-11T16:12:56Z
dc.date.issued2019-11-25
dc.identifier.urihttps://doi.org/10.3390/nano9121685
dc.identifier.urihttps://repository.tcu.edu/handle/116099117/39766
dc.identifier.urihttps://www.mdpi.com/2079-4991/9/12/1685
dc.description.abstractAlthough conventional antibiotics have evolved as a staple of modern medicine, increasing antibiotic resistance and the lack of antibiotic efficacy against new bacterial threats is becoming a major medical threat. In this work, we employ single-walled carbon nanotubes (SWCNTs) known to deliver and track therapeutics in mammalian cells via intrinsic near-infrared fluorescence as carriers enhancing antibacterial delivery of doxycycline and methicillin. SWCNTs dispersed in water by antibiotics without the use of toxic bile salt surfactants facilitate efficacy enhancement for both antibiotics against Staphylococcus epidermidis strain showing minimal sensitivity to methicillin. Doxycycline to which the strain did not show resistance in complex with SWCNTs provides only minor increase in efficacy, whereas the SWCNTs/methicillin complex yields up to 40-fold efficacy enhancement over antibiotics alone, suggesting that SWCNT-assisted delivery may circumvent antibiotic resistance in that bacterial strain. At the same time SWCNT/antibiotic formulations appear to be less toxic to mammalian cells than antibiotics alone suggesting that nanomaterial platforms may not restrict potential biomedical applications. The improvement in antibacterial performance with SWCNT delivery is tested via 3 independent assays--colony count, MIC (Minimal Inhibitory Concentration) turbidity and disk diffusion, with the statistical significance of the latter verified by ANOVA and Dunnett's method. The potential mechanism of action is attributed to SWCNT interactions with bacterial cell wall and adherence to the membrane, as substantial association of SWCNT with bacteria is observed--the near-infrared fluorescence microscopy of treated bacteria shows localization of SWCNT fluorescence in bacterial clusters, scanning electron microscopy verifies SWCNT association with bacterial surface, whereas transmission electron microscopy shows individual SWCNT penetration into bacterial cell wall. This work characterizes SWCNTs as novel advantageous antibiotic delivery/imaging agents having the potential to address antibiotic resistance.
dc.language.isoenen_US
dc.publisherMultidisciplinary Digital Publishing Institute
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceNanomaterials
dc.subjectcarbon nanotubes
dc.subjectantibiotic
dc.subjectmethicillin
dc.subjectdoxycycline
dc.subjectantibiotic delivery
dc.subjectfluorescence imaging
dc.subjectantibiotic resistance
dc.titleSingle-Walled Carbon Nanotube-Assisted Antibiotic Delivery and Imaging in S. epidermidis Strains Addressing Antibiotic Resistance
dc.typeArticle
dc.rights.holderKhazi-Syed et al.
dc.rights.licenseCC BY 4.0
local.collegeCollege of Science and Engineering
local.departmentPhysics and Astronomy
local.departmentChemistry and Biochemistry
local.personsKhazi-Syed, Hasan, Campbell, Naumov (PHYS); Gonzalez-Rodriguez (CHEM)


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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/