|Abstract||Background Capsaicin is the biologically active, spicy flavor profile component of chili peppers that has been recently touted as an anti-obesity agent. However, studies examining the effects of capsaicin on these markers have mixed results. Objective The purpose of this pilot study was to examine the effects of consuming a 14-d supply of 500mg/day or either capsaicin supplement versus placebo on: 1) basal metabolic rate (BMR); 2) blood glucose (BG); and 3) anthropometrics in college-aged men with BMI >25kg/m2. Design This study utilized a randomized, double-blind, placebo-controlled crossover design. Methods Six overweight/obese, sedentary men completed four visits (~45min/visit) over a 45-day intervention period. On visit 1, participants completed anthropometric and BMR measurements and were randomly assigned to either capsaicin or placebo. Participants were provided with a 14d supply of pills, a pill log, and dietary logs to take and complete daily for 14d. On day 15 (V#2), the same testing and measurements occurred. Participants then completed a 14-day washout period. Following the washout period, participants crossed-over and underwent the V#3 (days 30) and V#4 (days 45) where the same procedures as before were followed. Results From pre- to post-capsaicin supplementation, there were no significant changes in BMR (1.61±0.49 to 1.80±0.54 kcals/min, ns), BG (102.5±5.9 to 104.0±8.4mg/dL, ns), body weight (96.1±20.1 to 96.4±20.94kgs, ns), or BF% (22.2±9.2 to 22.7±8.6%, ns). Placebos showed no change in these markers (ns). Conclusions In overweight/obese college-aged men, supplementation with 500mg of capsaicin or placebo did not differentially affect BMR, BG or body composition. Overall, more research should ensue with a larger sample.