2025-06-032025-06-032025-05-19https://repository.tcu.edu/handle/116099117/67110Alzheimer's disease (AD) affects millions worldwide and has shown increasing prevalence. Over the last few years, it has become increasingly apparent that oxidative stress from reactive oxygen species (ROS) resulting in cell death and chronic inflammation plays a crucial role in AD disease progression along with other neurodegenerative diseases. The need for therapeutics to mitigate ROS and neuron death in the central nervous system (CNS) is evident to combat oxidative stress in the brain for AD and other neurodegenerative diseases. To investigate oxidative stress and potential neurotherapeutics, oxidative stress models in mouse neuron HT-22 cells have been developed. Glutamate treatment on HT-22 cells has been demonstrated to cause oxidative stress and cell death. By utilizing these cell lines, we can investigate the effects of oxidative stress and test with novel compounds to investigate their ability to resist oxidative stress and neuronal cell death.