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Iron-Regulated Phospholipase C Activity Contributes to the Cytolytic Activity and Virulence of Acinetobacter baumannii
Fiester, Steven E. Arivett, Brock A. Schmidt, Robert E. Beckett, Amber C. Ticak, Tomislav Carrier, Mary V. Ghosh, Rajarshi Ohneck, Emily J. Metz, Maeva L. Jeffries, Marlo K. Sellin
Fiester, Steven E.
Arivett, Brock A.
Schmidt, Robert E.
Beckett, Amber C.
Ticak, Tomislav
Carrier, Mary V.
Ghosh, Rajarshi
Ohneck, Emily J.
Metz, Maeva L.
Jeffries, Marlo K. Sellin
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Public Library of Science
Date
2016-11-22
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Abstract
Acinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound and urinary tract infections. Analysis of A. baumannii representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated that this pathogen is increasingly hemolytic to sheep, human and horse erythrocytes, which interestingly contain increasing amounts of phosphatidylcholine in their membranes. Bioinformatic, genetic and functional analyses of 19 A. baumannii isolates showed that the genomes of each strain contained two phosphatidylcholine-specific phospholipase C (PC-PLC) genes, which were named plc1 and plc2. Accordingly, all of these strains were significantly hemolytic to horse erythrocytes and their culture supernatants tested positive for PC-PLC activity. Further analyses showed that the transcriptional expression of plc1 and plc2 and the production of phospholipase and thus hemolytic activity increased when bacteria were cultured under iron-chelation as compared to iron-rich conditions. Testing of the A. baumannii ATCC 19606T plc1
Contents
Subject
Red blood cells
Phospholipases
Horses
Larvae
Gene expression
Transcriptional control
Acinetobacter baumannii
Intracellular pathogens
Phospholipases
Horses
Larvae
Gene expression
Transcriptional control
Acinetobacter baumannii
Intracellular pathogens
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Research Projects
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Journal Issue
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Description
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Department
Biology