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dc.creatorFiester, Steven E.
dc.creatorArivett, Brock A.
dc.creatorSchmidt, Robert E.
dc.creatorBeckett, Amber C.
dc.creatorTicak, Tomislav
dc.creatorCarrier, Mary V.
dc.creatorGhosh, Rajarshi
dc.creatorOhneck, Emily J.
dc.creatorMetz, Maeva L.
dc.creatorJeffries, Marlo K. Sellin
dc.creatorActis, Luis A.
dc.date.accessioned2017-06-29T16:32:08Z
dc.date.available2017-06-29T16:32:08Z
dc.date.issued2016-11-22
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0167068
dc.identifier.urihttps://repository.tcu.edu/handle/116099117/19797
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167068
dc.description.abstractAcinetobacter baumannii is an opportunistic Gram-negative pathogen that causes a wide range of infections including pneumonia, septicemia, necrotizing fasciitis and severe wound and urinary tract infections. Analysis of A. baumannii representative strains grown in Chelex 100-treated medium for hemolytic activity demonstrated that this pathogen is increasingly hemolytic to sheep, human and horse erythrocytes, which interestingly contain increasing amounts of phosphatidylcholine in their membranes. Bioinformatic, genetic and functional analyses of 19 A. baumannii isolates showed that the genomes of each strain contained two phosphatidylcholine-specific phospholipase C (PC-PLC) genes, which were named plc1 and plc2. Accordingly, all of these strains were significantly hemolytic to horse erythrocytes and their culture supernatants tested positive for PC-PLC activity. Further analyses showed that the transcriptional expression of plc1 and plc2 and the production of phospholipase and thus hemolytic activity increased when bacteria were cultured under iron-chelation as compared to iron-rich conditions. Testing of the A. baumannii ATCC 19606T plc1
dc.language.isoenen_US
dc.publisherPublic Library of Science
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourcePLoS One
dc.subjectRed blood cells
dc.subjectPhospholipases
dc.subjectHorses
dc.subjectLarvae
dc.subjectGene expression
dc.subjectTranscriptional control
dc.subjectAcinetobacter baumannii
dc.subjectIntracellular pathogens
dc.titleIron-Regulated Phospholipase C Activity Contributes to the Cytolytic Activity and Virulence of Acinetobacter baumannii
dc.typeArticle
dc.rights.holderSteven E. Fiester et al.
dc.rights.licenseCC BY 4.0
local.collegeCollege of Science and Engineering
local.departmentBiology
local.personsJeffries (BIOL)


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