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Preparation of Bacterial Fatty Acid Methyl Esters for Rapid Characterization by Gas-Liquid Chromatography
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American Society for Microbiology
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11/1/1967
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Abstract
Our recent survey (B. L. Brian and E. W. Gardner, unpublished data) of fatty acids extracted from a variety of bacterial species (Escherichia coli, Staphylococcus aureus, Sarcina lutea, Serratia marcescens, Salmonella shottmuelleri, Proteus vulgaris, Bacillus subtilis, Pseudomonas fluorescens, and several strains of Vibrio cholerae) called for a rapid, reproducible technique for extraction, esterification, and analysis of these substances. An extraction procedure was devised; several esterification methods were compared by gas-liquid chromatographic analysis and are here described.
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BrianPreparation.pdf
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Biology