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One of these strains is not like the others: C. elegans DW102 has an altered dependence on brc-1 and brd-1 for regulation of cyp gene transcription
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Caltech Library
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6/4/2024
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Abstract
Several strains of Caenorhabditis elegans with mutations in brc-1 or brd-1 are readily available to aid in elucidating the functions of these two genes in DNA damage repair, meiosis, and gene repression. DW102 is the only C. elegans strain to our knowledge with mutations in both brc-1 and brd-1. However, several groups have reported the DW102 strain is indistinguishable from wild-type when observing levels of embryonic lethality, sensitivity to radiation, and rates of male progeny, while strains with mutations in either brc-1 or brd-1 display increased occurrence of these phenotypes. Here, RT-qPCR analysis of the cyp-13A gene family, reveals distinctive and aberrant expression patterns in DW102 compared to other brc-1 or brd-1 mutant strains underscoring the need for caution in choosing this strain to draw conclusions about brc-1 and brd-1 functions. The expression profiles of cyp-13A2, cyp-13A4, cyp-13A5, cyp-13A6, cyp-13A7, cyp-13A8, cyp-13A10, cyp-13A11, and cyp-13A12 in wild-type N2 (WT) and DW102 strains were assessed using RT-qPCR and normalized to the expression of the reference gene, tba-1. Data are presented as the mean starting quantity relative to WT. Error bars represent the standard error of the mean (� SEM) for four biological replicates. The deviation from WT was determined using student's t-test with * indicating a statistically significant value (p-value < 0.05).
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ThapaOne.pdf
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Biology