Fixation and Histological Techniques for the Rat Model of Alzheimer's DiseaseShow full item record
Title | Fixation and Histological Techniques for the Rat Model of Alzheimer's Disease |
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Author | Jones, Reagan |
Date | 2022 |
Abstract | Alzheimer's disease (AD) is a progressive form of dementia characterized by severe memory loss and protein anomalies in the brain, the severity of which is strongly correlated with a variety of genetic and environmental factors currently being studied. Animal models of AD allow for precise investigation of pathological mechanisms involved in the disease process, which provides valuable insight into potential etiology, treatments, and early indicators of the disease. This project focuses specifically on the Formalin-Fixed Paraffin-Embedded (FFPE) tissue processing method for the analysis of Alzheimer's disease-like pathology in the brains of Tg-F344 rats. These animal subjects express mutant human amyloid precursor protein (APPsw) and presenilin 1 (PS1dE9) genes, both of which have been found to drive amyloid beta accumulation and cause early-onset familial AD in humans. Modification of the reagents and techniques used for embedding, sectioning, and staining tissue in this method can greatly affect the integrity and morphology of the samples being studied. The lack of a standardized, universal FFPE protocol due to the varying nature of sample tissues and instruments requires that techniques be adapted to suit the needs of individual laboratories. The goal of the current study is to identify an efficient and effective method to obtain well-preserved rat brain tissue samples that are readily available for histochemistry and analysis in our laboratory, as this is essential to understanding the underlying pathology of AD and how it correlates to genetic and environmental factors. |
Link | https://repository.tcu.edu/handle/116099117/54225 |
Department | Biology |
Advisor | Chumley, Michael |
Additional Date(s) | 5/19/2022 |
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- Undergraduate Honors Papers [1362]
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