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A homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environmentsShow simple item record

dc.creatorRaut, Sangram L.
dc.creatorKimball, Joseph D.
dc.creatorFudala, Rafal
dc.creatorDoan, Hung
dc.creatorMaliwal, Badri P.
dc.creatorSabnis, Nirupama A.
dc.creatorLacko, Andras G.
dc.creatorGryczynski, Ignacy
dc.creatorDzyuba, Sergei V.
dc.creatorGryczynski, Zygmunt
dc.date.accessioned2016-08-03T21:49:56Z
dc.date.available2016-08-03T21:49:56Z
dc.date.issued2014-10-29
dc.identifier.urihttps://doi.org/10.1039/C4CP04260C
dc.identifier.urihttps://repository.tcu.edu/handle/116099117/11224
dc.identifier.urihttps://pubs.rsc.org/en/Content/ArticleLanding/2014/CP/C4CP04260C
dc.description.abstractFluorescence properties of a novel homodimeric BODIPY dye rotor for Fluorescence Lifetime Imaging Microscopy (FLIM) are reported. Steady state and time resolved fluorescence measurements established the viscosity dependent behaviour in vitro. Homodimeric BODIPY embedded in different membrane mimicking lipid vesicles (DPPC, POPC and POPC plus cholesterol) is demonstrated to be a viable sensor for fluorescence lifetime based viscosity measurements. Moreover, SKOV3 cells readily endocytosed the dye, which accumulated in membranous structures inside the cytoplasm thereby allowing viscosity mapping of internal cell components.
dc.language.isoenen_US
dc.publisherThe Royal Society of Chemistry
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.sourcePhysical Chemistry Chemical Physics
dc.subjectmolecular rotors
dc.subjectlive cells
dc.subjectdyes
dc.subjectchemistry
dc.titleA homodimeric BODIPY rotor as a fluorescent viscosity sensor for membrane-mimicking and cellular environments
dc.typeArticle
dc.rights.holderRaut et al.
dc.rights.licenseCC BY 3.0
local.collegeCollege of Science and Engineering
local.departmentPhysics and Astronomy
local.departmentChemistry and Biochemistry
local.personsRaut, Kimball, Doan, Gryczynski (PHYS); Dzyuba (CHEM)


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