Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells

Requena, Sebastian; Ponomarchuk, Olga; Castillo, Marlius K.; Rebik, Jonathan; Brochiero, Emmanuelle; Borejdo, Julian; Gryczynski, Ignacy; Dzyuba, Sergei V.; Gryczynski, Zygmunt; Grygorczyk, Ryszard; Fudala, Rafal

dc.creator	Requena, Sebastian
dc.creator	Ponomarchuk, Olga
dc.creator	Castillo, Marlius K.
dc.creator	Rebik, Jonathan
dc.creator	Brochiero, Emmanuelle
dc.creator	Borejdo, Julian
dc.creator	Gryczynski, Ignacy
dc.creator	Dzyuba, Sergei V.
dc.creator	Gryczynski, Zygmunt
dc.creator	Grygorczyk, Ryszard
dc.creator	Fudala, Rafal
dc.date.accessioned	2019-07-11T15:17:10Z
dc.date.available	2019-07-11T15:17:10Z
dc.date.issued	2017-12-01
dc.identifier.uri	https://doi.org/10.1038/s41598-017-17037-2
dc.identifier.uri	https://repository.tcu.edu/handle/116099117/26385
dc.identifier.uri	https://www.nature.com/articles/s41598-017-17037-2
dc.description.abstract	Abnormalities of mucus viscosity play a critical role in the pathogenesis of several respiratory diseases, including cystic fibrosis. Currently, there are no approaches to assess the rheological properties of mucin granule matrices in live cells. This is the first example of the use of a molecular rotor, a BODIPY dye, to quantitatively visualize the viscosity of intragranular mucin matrices in a large population of individual granules in differentiated primary bronchial epithelial cells using fluorescence lifetime imaging microscopy.
dc.language.iso	en	en_US
dc.publisher	Nature Publishing Group
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.source	Scientific Reports
dc.subject	Biophysics
dc.subject	Chemical tools
dc.title	Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells
dc.type	Article
dc.rights.holder	Requena et al.
dc.rights.license	CC BY 4.0
local.college	College of Science and Engineering
local.department	Chemistry and Biochemistry
local.department	Physics and Astronomy
local.persons	Castillo, Rebik, Dzyuba (CHEM); Gryczynski (PHYS)