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dc.creatorAjgaonkar, Ryan
dc.creatorLee, Bong
dc.creatorValimukhametova, Alina
dc.creatorNguyen, Steven
dc.creatorGonzalez-Rodriguez, Roberto
dc.creatorCoffer, Jeffery
dc.creatorAkkaraju, Giridhar R.
dc.creatorNaumov, Anton V.
dc.date.accessioned2023-01-18T21:37:22Z
dc.date.available2023-01-18T21:37:22Z
dc.date.issued2022
dc.identifier.urihttps://doi.org/10.3390/ma15165760
dc.identifier.urihttps://repository.tcu.edu/handle/116099117/56998
dc.description.abstractEarly-stage pancreatic cancer remains challenging to detect, leading to a poor five-year patient survival rate. This obstacle necessitates the development of early detection approaches based on novel technologies and materials. In this work, the presence of a specific pancreatic cancer-derived miRNA (pre-miR-132) is detected using the fluorescence properties of biocompatible nitrogen-doped graphene quantum dots (NGQDs) synthesized using a bottom-up approach from a single glucosamine precursor. The sensor platform is comprised of slightly positively charged (1.14 ± 0.36 mV) NGQDs bound via ??? stacking and/or electrostatic interactions to the negatively charged (?22.4 ± 6.00 mV) bait ssDNA; together, they form a complex with a 20 nm average size. The NGQDs’ fluorescence distinguishes specific single-stranded DNA sequences due to bait–target complementarity, discriminating them from random control sequences with sensitivity in the micromolar range. Furthermore, this targetability can also detect the stem and loop portions of pre-miR-132, adding to the practicality of the biosensor. This non-invasive approach allows cancer-specific miRNA detection to facilitate early diagnosis of various forms of cancer.en_US
dc.language.isoen_USen_US
dc.publisherMDPI AG
dc.sourceMaterials
dc.titleDetection of Pancreatic Cancer miRNA with Biocompatible Nitrogen-Doped Graphene Quantum Dots
dc.typeArticleen_US
dc.rights.licenseCC BY 4.0
local.collegeCollege of Science and Engineering
local.departmentBiology
local.departmentPhysics and Astronomy
local.departmentChemistry and Biochemistry
local.personsAjgaonkar, Akkaraju (BIOL), Lee, Valimukhuametova, Nguyen, Naumov (PHYS), Coffer (CHEM)


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