| dc.creator | Kapic, Ammar | |
| dc.creator | Sabnis, Nirupama | |
| dc.creator | Dossou, Akpedje S. | |
| dc.creator | Chavez, Jose | |
| dc.creator | Ceresa, Luca | |
| dc.creator | Gryczynski, Zygmunt | |
| dc.creator | Fudala, Rafal | |
| dc.creator | Dickerman, Rob | |
| dc.creator | Bunnell, Bruce A. | |
| dc.creator | Lacko, Andras G. | |
| dc.date.accessioned | 2024-09-25T21:35:57Z | |
| dc.date.available | 2024-09-25T21:35:57Z | |
| dc.date.issued | 7/5/2024 | |
| dc.identifier.uri | https://doi.org/10.3390/ijms25137378 | |
| dc.identifier.uri | https://repository.tcu.edu/handle/116099117/65965 | |
| dc.description | Cytotoxic activity has been reported for the xanthone alpha-mangostin (AMN) against Glioblastoma multiforme (GBM), an aggressive malignant brain cancer with a poor prognosis. Recognizing that AMN's high degree of hydrophobicity is likely to limit its systemic administration, we formulated AMN using reconstituted high-density lipoprotein (rHDL) nanoparticles. The photophysical characteristics of the formulation, including fluorescence lifetime and steady-state anisotropy, indicated that AMN was successfully incorporated into the rHDL nanoparticles. To our knowledge, this is the first report on the fluorescent characteristics of AMN with an HDL-based drug carrier. Cytotoxicity studies in a 2D culture and 3D spheroid model of LN-229 GBM cells and normal human astrocytes showed an enhanced therapeutic index with the rHDL-AMN formulation compared to the unincorporated AMN and Temozolomide, a standard GBM chemotherapy agent. Furthermore, treatment with the rHDL-AMN facilitated a dose-dependent upregulation of autophagy and reactive oxygen species generation to a greater extent in LN-229 cells compared to astrocytes, indicating the reduced off-target toxicity of this novel formulation. These studies indicate the potential therapeutic benefits to GBM patients via selective targeting using the rHDL-AMN formulation. | |
| dc.language | en | |
| dc.publisher | MDPI AG | |
| dc.source | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | |
| dc.title | Photophysical Characterization and In Vitro Evaluation of a-Mangostin-Loaded HDL Mimetic Nano-Complex in LN-229 Glioblastoma Spheroid Model | |
| dc.type | Article | |
| dc.rights.license | CC BY 4.0 | |
| local.college | College of Science and Engineering | |
| local.department | Physics and Astronomy | |
| local.persons | Chavez, Ceresa, Gryczynski (PHYS) | |
