Pro-inflammatory Cytokine Production by BV2 Microglial CellsShow full item record
|Pro-inflammatory Cytokine Production by BV2 Microglial Cells
|Alzheimer's Disease (AD) is a neurodegenerative disease that primarily affects elderly populations. AD engenders memory loss and cognitive decline, and its prevalence is rapidly growing. It is estimated that 14 million Americans will have AD by the year 2050. Therefore, it is imperative for researchers to examine the underlying biological mechanisms responsible for AD. Previous research has demonstrated that chronic inflammation is linked to the hallmark AD pathology, amyloid beta. Amyloid beta is a protein that disrupts neuronal communication and increases the production of effector proteins called pro-inflammatory cytokines. Microglia function like immune cells in the brain, and when they are activated by inflammatory triggers, such as amyloid beta, they secrete pro-inflammatory cytokines. Although cytokine release is initially a healthy response, excess cytokine production is harmful to the brain and exacerbates AD pathologies. Prior research has demonstrated that pro-inflammatory cytokines are upregulated in the serum of AD patients. Therefore, cytokines are a crucial target for AD therapeutics. This project examined the temporal inflammatory response of microglial cells following lipopolysaccharide (LPS) insult. LPS is a component of common bacteria and can induce inflammation in microglial cells. We treated cells with several different concentrations of LPS and assessed cytokine production at several different timepoints. Here we show that microglia treated with LPS produce cytokines in a dose- and time-dependent manner. In addition, the production of different cytokines does not follow the same temporal pattern. These data will help us pinpoint proper testing procedures for therapeutic compounds that are currently under development.
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- Undergraduate Honors Papers