Anti-Inflammatory Effect of CBD on Cultured MicrogliaShow full item record
Title | Anti-Inflammatory Effect of CBD on Cultured Microglia |
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Author | O'Connor, Caroline |
Date | 2022 |
Abstract | Alzheimer's Disease (AD) is a progressive neurodegenerative disease associated with old age and marked by deficits in memory and learning skills. AD pathology is characterized by amyloid-beta (AB) accumulation, which leads to plaque formation and ultimately neuronal death. Additionally, AB activates microglial cells, which function as an immune cell in the brain. Microglial cells secrete proteins that induce inflammation, known as pro-inflammatory cytokines. The chronic activation of pro-inflammatory cytokines engenders neuroinflammation and oxidative stress, which then further exacerbate AD pathologies. This project aims to study the effectiveness of cannabidiol (CBD) as a potential treatment for AD, due to its known anti-inflammatory properties. We measured the inflammatory response of cultured BV2 immortalized murine microglial cells following lipopolysaccharide (LPS) treatment. We then included a CBD treatment to study its therapeutic capabilities in reducing inflammation. We hypothesized that treatment with CBD would decrease the pro-inflammatory cytokines TNF-alpha and IL-6 induced by LPS stimulation. We performed enzyme-linked immunosorbent assays (ELISAs) to detect and quantify the cytokine levels. The overall goal of the research is to demonstrate the capacity of CBD to minimize the immunological mechanisms that drive AD pathologies. Our research will contribute to the understanding of the link between the immune system and central nervous system in AD development. AD is the sixth leading cause of death in America, but the availability of therapies is limited. CBD represents a natural and possibly effective therapy for those suffering from Alzheimer's disease, and our research will contribute to determining its efficacy. |
Link | https://repository.tcu.edu/handle/116099117/54209 |
Department | Biology |
Advisor | Chumley, Michael |
Additional Date(s) | 5/19/2022 |
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- Undergraduate Honors Papers [1362]
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